Protein Content of Madracis decactis and Diploria labyrinthiformis tissue for Physiological Analyses

This protocol was adapted from the proceedure provided by the Pierce BCA Protein Assay Kit from Thermo Scientific

Contents

• Samples
• Protocol
• Standard Table
• References

  20241212

  Run 1 (note that in the physical notebook run 1 is named Batch 1)

  |Sample|Volume of NaOH (μL)|Volume of HCL (μL)| Replicate 1 Plate Postion| Replicate 2 Plate Postion| |——|——————-|——————|————————–|————————–| |MD-2-21|10|80|A2|H11 |MD-2-7|10|65|D6|H7| |DL-4-25|10|65|G7|A7| |DL-3-9|10|65|H6|E10| |MD-1-13|10|65|D1|H4| |MD-1-5|10|60|B6|F11| |MD-1-24|10|85|E1|G12| |MD-5-3|10|60|A4|F9| |DL-1-17|10|55|B3|E8| |MD-1-14|10|40|C1|D11 |DL-1-15|10|65|F6|C10| |DL-3-18|10|60|C6|H9| |MD-2-19|10|40|G1|C12| |DL-2-22|10|70|A10|E11| |DL-1-11|10|50|D3|G10| |DL-3-14|10|70|A3|H10| |DL-3-6|10|70|A5|G8| |DL-2-24|10|80|B1|H8| |MD-1-20|10|80|H3|A8| |DL-1-6|10|70|F7|C2|

• Run 1 CURVE

• Curve Letter	Replicate 1 Plate Postion	Replicate 2 Plate Postion
  A	A1	H12
  B	B2	G11
  C	C3	F10
  D	D4	E9
  E	E5	F8
  F	F1	E12
  G	G2	F12
  H	H5	A9
  I	C5	G9

Run 2 (note that in the physical notebook run 2 is named Batch 2)

20240128

|Sample|Volume of NaOH (μL)|Volume of HCL (μL)| Replicate 1 Plate Postion| Replicate 2 Plate Postion| |——|——————-|——————|————————–|————————–| |MD-1-4|10| |DL-1-3|10| |DL-3-5|10| |MD-5-7|10| |MD-4-11|10| |DL-4-14|10| |MD-1-23|10| |MD-4-4|10| |DL-3-17|10| |MD-2-25|10| |MD-4-20|10| |MD-4-5|10| |MD-2-23|10| |DL-4-4|10| |MD-2-15|10| |DL-4-6|10| |DL-2-12|10| |DL-4-26|10| |MD-4-14|10| |MD-2-4|10| |MD-1-10|10| |MD-4-25|10| |MD-2-12|10|